Covalent Modification of the Flavin in Proline Dehydrogenase by Thiazolidine-2-Carboxylate

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Evidence for hysteretic substrate channeling in the proline dehydrogenase and Δ1-pyrroline-5-carboxylate dehydrogenase coupled reaction of proline utilization A (PutA).

PutA (proline utilization A) is a large bifunctional flavoenzyme with proline dehydrogenase (PRODH) and Δ(1)-pyrroline-5-carboxylate dehydrogenase (P5CDH) domains that catalyze the oxidation of l-proline to l-glutamate in two successive reactions. In the PRODH active site, proline undergoes a two-electron oxidation to Δ(1)-pyrroline-5-carboxlylate, and the FAD cofactor is reduced. In the P5CDH ...

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ژورنال

عنوان ژورنال: ACS Chemical Biology

سال: 2020

ISSN: 1554-8929,1554-8937

DOI: 10.1021/acschembio.9b00935